Coding

Part:BBa_K1067004:Design

Designed by: Kristian Davidsen and Helen Cook   Group: iGEM13_DTU-Denmark   (2013-09-23)

Hydroxylamine oxidoreductase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 811
    Illegal PstI site found at 1552
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 811
    Illegal PstI site found at 1552
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 811
    Illegal BamHI site found at 246
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 811
    Illegal PstI site found at 1552
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 811
    Illegal PstI site found at 1552
    Illegal NgoMIV site found at 2348
    Illegal NgoMIV site found at 2382
    Illegal AgeI site found at 270
    Illegal AgeI site found at 1970
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 328
    Illegal BsaI.rc site found at 709


Design Notes

Genes taken from Nitrosomonas europaea ATCC 19718 by colony PCR.

References